DREAM is a Dialogue for Reverse Engineering Assessments and Methods. The main objective is to catalyze the interaction between experiment and theory in the area of cellular network inference and quantitative model building in systems biology.

challenge1: perturbation experiments

Submitted by LBM on Wed, 2011-07-27 21:43

Hi,

Just to be sure, when selecting an mRNA knockdown perturbation experiment it results on 5-fold increase in mRNA degradation, ONLY for the specified gene, right? And similarly for Increase in RBS (i.e. double rbs_strength of the specified gene only).

If I'm right, then what does the "wildtype gene" option mean after selecting mRNA knockdown or RBS increase?

Thank you,

Zeina

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Hi Zeina Both for mRNA

Submitted by Dream_manager on Thu, 2011-07-28 00:49.

Hi Zeina

Both for mRNA degradation and changing the RBS, only the specified gene is directly affected.

The wildtype option is a default option that is useful ONLY when you perform measurements without modifying (i.e Gene deletion, mRNA knockdown , Increase of RBS activity) the cells.

For example: You might want the High-resolution mRNA expression data or more fluorescent data on p1 and p2.

Hope this clarifies

Pablo

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challenge1: perturbation experiments

Comments

Hi Zeina Both for mRNA